Ascorbyl palmitate is an efficient fat-soluble antioxidant and nutritional fortifier. It has been recognized by the World Health Organization as a safe and non-toxic food additive, and it is also the only antioxidant permitted for use in infant foods in China. However, excessive use may lead to adverse effects such as gastrointestinal irritation, tooth damage, and elevated blood pressure. Therefore, it is necessary to establish a reliable method for the determination of ascorbyl palmitate in food. Referring to GB 5009.308-2025 Determination of Ascorbyl Palmitate in Foods, this application determined the content of ascorbyl palmitate in branded cola and milk powder samples.
Conclusion
Using the Leaps UHPLC system and following GB 5009.308-2025 Determination of Ascorbyl Palmitate in Foods, a methodological evaluation was performed. The method showed excellent linearity, with a correlation coefficient greater than 0.9999. The repeatability of retention time was 0.11%, and the repeatability of peak area was 0.20%. At the spiking level corresponding to the limit of quantification, the recovery was 87.0% for milk powder and 85.5% for cola. The method is simple, stable, and highly sensitive, fully meeting the requirements for the determination of ascorbyl palmitate in food.
