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High performance liquid chromatography (HPLC) is an analytical chemistry technique used to separate compounds from chemical mixtures. These separations utilize the following principle: the liquid mobile phase flows under pressure through a chromatographic column containing a stationary phase.
HPLC instruments generally have four main hardware components: pump, automatic sampler, column temperature chamber, and detector. Many factors, including the composition of the mobile phase, the chemical composition of the stationary phase, and temperature, can affect HPLC separation. Only when the analyte has differential affinity for the stationary phase, will it be successfully separated. Therefore, selecting a suitable stationary phase for the compound is crucial.
HPLC has a wide range of applications. This includes testing the quality of the products you use every day. From drug impurity analysis, food and beverage ingredient quality identification, to monitoring pollutant levels in water and soil, HPLC can help ensure consumer safety.
HPLC has a wide range of applications. This includes testing the quality of the products you use every day. From drug impurity analysis, food and beverage ingredient quality identification, to monitoring pollutant levels in water and soil, HPLC can help ensure consumer safety.
This video introduces a brief history of chromatographic analysis and elucidates the fundamental principles behind HPLC and UHPLC
