Small interfering RNA (siRNA) therapeutics are among the most promising treatment approaches, offering high specificity, broad target range, and long-lasting effects. siRNA typically consists of double-stranded RNA sequences with 19–23 nucleotides, and chemical synthesis is the most direct and reliable method for their production.For purity and impurity analysis of siRNA, commonly used liquid chromatography methods include ion-pair reversed-phase chromatography and ion-exchange chromatography.In this study, the Chromai Leaps Pro Bio bio-inert LC system was used with anion-exchange chromatography to rapidly separate the sense strand, antisense strand, and related impurities in siRNA drugs.The results demonstrate that the system provides reliable performance, with peak shapes and analytical results comparable to those of leading international bio-inert systems.The Leaps Pro Bio system fully meets the requirements for bio-inert LC systems in nucleic acid drug analysis and can be widely applied to purity and impurity analysis of nucleic acid-based therapeutics.
Leaps Pro Bio Bio-inert LC System
Enables efficient analysis of biological samples
True bio-inert flow path design
Corrosion-resistant and low-adsorption system
Suitable for biological and extreme pH applications
Ensures stable and reliable analysis
Advanced Bio-inert Design
1
PEEK, titanium, and other bio-compatible materials
2
Suitable for biological and extreme pH conditions
3
Minimizes sample loss caused by metal adsorption
Advanced Sample Handling
1
Efficient integrated cooling system (4°C to ambient)
2
Expandable to 4 sample trays (216 or 384 positions)
3
High reproducibility with no sample loss
