Yinqiao Jiedu Granules are composed of 9 traditional Chinese medicinal materials including Lonicerae Japonicae Flos, Forsythiae Fructus, Menthae Haplocalycis Herba, Schizonepetae Herba, etc. They are a collection item in the Pharmacopoeia of the People’s Republic of China and have the effects of relieving exterior syndrome with pungent and cool-natured drugs, and clearing away heat and detoxifying. They are used for wind-cold cold, headache and fever, aversion to cold and body pain, cough and dry throat.
In accordance with the method for the determination of chlorogenic acid in Yinqiao Jiedu Granules specified in the Pharmacopoeia of the People’s Republic of China (2020 Edition), this paper determined the active ingredient chlorogenic acid in Yinqiao Jiedu Granules and carried out methodological validation. The experimental results show that the determination of chlorogenic acid in Yinqiao Jiedu Granules using the Chromai Leaps UHPLC system fully meets the requirements of the Pharmacopoeia of the People’s Republic of China, with an accurate and sensitive method.
??Experimental Instruments
Chromai Leaps Ultra-High Performance Liquid Chromatography System
??Conclusion
In this study, based on the chlorogenic acid peak, the theoretical plate number was 10468 (the pharmacopoeia requirement is no less than 3000). Each bag of Yinqiao Jiedu Granules contains 6.4 mg of chlorogenic acid calculated as Lonicerae Japonicae Flos (the pharmacopoeia requires no less than 6 mg per bag). The qualitative repeatability was 0.096%, quantitative repeatability 0.255%, and the linear range was 0.03 μg～0.3 μg (r=0.9999).
All test results fully meet the standard requirements for Yinqiao Jiedu Granules in the Pharmacopoeia of the People’s Republic of China. In addition, the instrument features a stable baseline and low noise, fully satisfying analytical requirements.
