Ganmao Qingre Granules are composed of 11 traditional Chinese medicinal herbs, including Schizonepeta, mint, Saposhnikovia, Bupleurum, perilla, pueraria, platycodon, bitter apricot seed, angelica dahurica, taraxacum, and reed rhizome. They are included in the Chinese Pharmacopoeia and are used for dispelling wind and cold, relieving exterior syndromes, and clearing heat. Clinically, they are used to treat wind-cold type common cold, characterized by headache, fever, chills, body aches, clear nasal discharge, cough, and dry throat.
According to the Chinese Pharmacopoeia (2020 edition), this study determines the content of puerarin, an active component in Ganmao Qingre Granules, and performs method validation.The results demonstrate that the determination of puerarin using the Chromai Leaps UHPLC system fully meets the requirements of the Chinese Pharmacopoeia, with excellent accuracy and sensitivity.
Instrument：Chromai Leaps UHPLC System
Conclusion：
Theoretical plate number calculated based on the puerarin peak was 10,293 (Pharmacopoeia requirement: not less than 4,500).The content of puerarin per sachet in Ganmao Qingre Granules was 11.27 mg (Pharmacopoeia requirement: not less than 10 mg per sachet).The linear range of puerarin was 43 μg to 215 μg (r = 0.9999).All test results comply with the standards specified in the Chinese Pharmacopoeia for Ganmao Qingre Granules.In addition, the instrument demonstrated a stable baseline with low noise, fully meeting analytical requirements.
