Fat-soluble vitamins A, D and E are essential nutrients for the human body to maintain normal metabolism and physiological functions. Fortified foods and foods for special medical purposes serve as important ways of vitamin supplementation. Accurate determination of vitamin contents in such foods is of great significance for scientific dietary guidance and food safety assurance. In practical analysis, however, these sample matrices are highly complex and cause severe interference in conventional chromatographic analysis, thereby requiring cumbersome sample pretreatment procedures.
National Food Safety Standard GB 5009.296-2023 Determination of Vitamin D in Foods has for the first time incorporated the on-line column-switching reversed-phase liquid chromatography as a standard method, providing a superior reference for the determination of vitamin D. On this basis, Chromai Technology integrates online solid phase extraction (Online SPE) into two-dimensional liquid chromatography (2D-LC), realizing the technological upgrade of sample pretreatment and delivering a substantial improvement in analytical efficiency for customer laboratories.
Referring to the group standard T/SHFCA 002-2024, this protocol establishes a method for the determination of vitamin A, D and E in formula milk powder by adopting Chromai online solid phase extraction coupled with heart-cutting two-dimensional liquid chromatography.
This solution adopts Chromai dedicated chromatographic columns for vitamin A/D/E analysis, achieving effective separation of vitamin A, four isomers of vitamin E, as well as vitamin D2 and D3. The application of online solid phase extraction technology greatly simplifies the sample pretreatment procedure, improves detection efficiency and reduces manual errors. Equipped with Chromai’s high-sensitivity full-reflection diode array detector, accurate quantitative analysis of low-level vitamin D can be realized. In addition, the method exhibits good reproducibility and stability in preliminary durability tests. It can be popularized and applied as an efficient and reliable approach for the determination of vitamin A, D and E in infant formula milk powder.
