Taurine, also known as 2-aminoethanesulfonic acid, is a sulfur-containing non-protein amino acid.Taurine plays an important role in regulating physiological functions, including anti-inflammatory and analgesic effects, maintaining osmotic balance, supporting vision, enhancing immunity, antioxidant activity, and protecting myocardial cells.The Chinese National Food Safety Standard GB 5009.169-2016 specifies methods for determining taurine in various food products, including infant formula, dairy products, beverages, and other food categories.
This application follows Method I of the standard—HPLC with post-column derivatization using o-phthalaldehyde (OPA). Separation is performed using an ion-exchange column, followed by post-column derivatization with OPA, and detection using a fluorescence detector to accurately determine taurine content in beverages.
Quaternary Gradient Pump P40, Autosampler A10C, Column Oven C10, Fluorescence Detector D50, Post-column Derivatization System R10
This method, based on the Chromai Leaps post-column derivatization system and aligned with GB 5009.169-2016, establishes a reliable method for determining taurine in beverages.The taurine calibration curve showed excellent linearity with a correlation coefficient of 1.0000.Retention time repeatability was 0.03%, and peak area repeatability was 0.09%.The detection limit meets national standard requirements, and recovery ranges from 85% to 100%.This method can be used as a reliable analytical approach for determining taurine content in beverages.
