Deep component analysis empowers material research and quality upgrading
Chemical materials
In short, excessive or long-term exposure to FWAs may have various adverse effects on human health. To minimize potential health risks, the public should raise health awareness and choose products with little or no FWA content. Thus, it is necessary to develop a method capable of simultaneously detecting multiple FWAs.
This solution is based on the Chinese Textile Industry Standard FZ/T 01137-2016, establishing an HPLC-fluorescence detection method for simultaneous quantification of six FWAs.
Conclusion
This method uses the CHROMAI Leaps UHPLC - Fluorescence Detector to simultaneously determine the content of six FWAs (220, 85, 71, 162, 199, 135) in face masks. All compounds achieved baseline separation with good linearity (R > 0.999), and the recovery rate in actual spiked samples ranged from 90.3% to 112.8%. This method provides a fast, efficient, and reliable solution for detecting FWAs in masks and other textile products.
Arbutin appears as white needle - like crystals or powder. It can inhibit the activity of tyrosinase in the body, prevent the formation of melanin, and has a good whitening effect. Therefore, it is often used in whitening cosmetics. α- Arbutin and β- arbutin are isomers with different whitening effects, and both can be converted into hydroquinone under certain conditions. Deoxyarbutin has a strong antioxidant effect and can also effectively inhibit the activity of tyrosinase. It can be converted into hydroquinone under room temperature, weak acid conditions, or when exposed to light in an aqueous solution. The European Commission's Regulation (EU) 2021/1099 prohibits the use of deoxyarbutin in cosmetics. Hydroquinone and phenol also have good whitening effects, but due to their high toxicity and strong irritation, long - term exposure may pose a carcinogenic risk. In China's "Technical Specifications for Cosmetic Safety" (2015 Edition), both are listed as prohibited raw materials for cosmetics. Therefore, it is necessary to establish a method for the simultaneous determination of these five compounds.
Currently, the detection methods for these compounds mainly include micellar electrokinetic capillary chromatography, high - performance liquid chromatography - diode array detection, gas chromatography, gas chromatography - tandem mass spectrometry, etc. Usually, only 1-3 of these substances can be detected, and deoxyarbutin has not been detected. This application simultaneously analyzes these five compounds by high - performance liquid chromatography - fluorescence detector.
Instruments：UHPLC（P40 - Quaternary gradient pump、A10 - Autosampler、C10 - Column Oven、D50 - Flourescence detector）
Conclusion
This method uses Chromai’s UHPLC - fluorescence detector to simultaneously determine the contents of α- arbutin, β- arbutin, deoxyarbutin, hydroquinone, and phenol in cosmetics. The method has a good linear relationship. The RSD% of the retention time of each compound is less than 0.2%, and the RSD% of the peak area is less than 0.4%. There is no interference at the target peak of the actual sample (facial cream). The spiked recovery of the actual sample is 93.9% - 98.1%. The detection limit and quantitation limit meet the detection requirements.
In cosmetics, thioglycolic acid, glycerol thioglycolate, methyl thioglycolate, dithiodiglycolic acid, ethyl thioglycolate, isopropyl thioglycolate, butyl thioglycolate, and isooctyl thioglycolate have exfoliating, moisturizing, and beautifying effects, and can also reduce the appearance of dark spots and pigmentation. However, long-term or excessive use of cosmetics containing these ingredients may irritate the skin and cause allergic reactions.
This application is based on the Safety and Technical Standards for Cosmetics (2015 Edition) and uses the Leaps Duo Parallel Liquid Chromatography System to divide the eight thioglycolic compounds in cosmetics into two groups and analyze them simultaneously under different chromatographic conditions.
Conclusion
In this application, the Leaps Duo Parallel Liquid Chromatography System from Chromai Technologies was used to establish a rapid method for the determination of eight thioglycolic compounds in cosmetics.
The results showed:
- ?Correlation coefficients (R2) greater than 0.9997 for all compounds;
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- ?Retention time RSD% < 1%, and peak area RSD% < 1.5%;
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- ?Spiked recovery rates between 88.85% and 102.50%.
This method demonstrates a high degree of automation, excellent stability, high sensitivity, and simple operation.By utilizing a dual-injection system, the analysis time was reduced by half, significantly improving efficiency.It is suitable for the rapid determination of thioglycolic compounds in cosmetic products.
Formaldehyde may be present in cosmetics, usually in trace amounts, primarily released from preservatives rather than added directly.According to regulatory requirements, the maximum permitted concentration of formaldehyde in cosmetics is 0.2%. While formaldehyde used within compliance limits poses a low carcinogenic risk, it can still cause allergic reactions in certain individuals.In addition, some toothpastes contain formaldehyde-releasing agents, which can slowly release formaldehyde under certain conditions. Although the amount is small, long-term use may still harm the body.Therefore, it is advisable to choose cosmetics and toothpastes with low or no formaldehyde content.
In this application, following the 2015 edition of the “Cosmetic Safety Technical Specification,” a certain brand of facial toner and toothpaste was analyzed using both pre-column derivatization and post-column derivatization methods, and the results of the two methods were compared.
Conclusion
In this application, the Konomei Leaps UHPLC–UV detector was used to determine formaldehyde content via pre-column and post-column derivatization methods. Both methods showed good linearity with correlation coefficients greater than 0.9999.For real samples, the recovery rates for toothpaste using both derivatization methods ranged from 100.56% to 103.00%, while the recovery rates for cosmetic water ranged from 101.20% to 101.79%.The LODs for pre-column and post-column derivatization were 10 mg/kg and 30 mg/kg, respectively, both meeting the requirements of the “Cosmetic Safety Technical Specification.”The pre-column derivatization method had a lower LOD due to better derivatization conditions and reagent selection, but required manual derivatization, making it more prone to human error.The post-column derivatization method was more automated and provided more stable detection results.