It is applicable to the pretreatment of all biological samples separated and detected by liquid chromatography technologies (HPLC-MS/MS, HPLC-DAD, HPLC-UV, HPLC-FLD, HPLC-ECD, etc.), covering pretreatment operations for biological matrices such as whole blood, serum, plasma, urine, tissues, cerebrospinal fluid, sweat and saliva in pharmaceutical-related fields including in vitro diagnostics, clinical research and drug development.